Inhibition of Mycosphaerella fijiensis in organic banana (Musa AAA L.) with the application of plant growth-promoting bacteria
DOI:
https://doi.org/10.53287/kxys3855fa98oKeywords:
biocontrol, organic agriculture, polymerase chain reaction, Black SigatokaAbstract
The cultivation of organic bananas (Musa AAA L.) contributes to the livelihood of families, and to the economy through exports in the Dominican Republic. Exports of agricultural products have limitations; these include the maximum permitted limit of chemical residues that are present. Other constraints on production are the incidence of pests and diseases at the leaf level. The main banana disease is Sigatoka (Mycosphaerella spp.), the commercially important species being fijiensis. In organic plantations, biological alternatives are used as disease control and PGPRs bacteria are an alternative for promoting plant development and controlling pathogens. The aim of the research was to evaluate the effect of PGPRs bacteria on the development of Mycosphaerella fijiensis in vitro. Twenty bacterial strains with PGPRs capabilities were used for the assay. The fungus was isolated and identified by polymerase chain reaction (PCR). The in vitro assay was carried out in potato dextrose agar (PDA) culture medium in a Petri dish with three replicates per treatment, seeding in the center of the dish a disc of each bacterium that was allowed to grow for 24 hours at 28 °C. After some time, the fungus was inoculated with an approximate concentration of 1x105 FM ml. The assay was incubated at a temperature of 28 ºC for 14 days. The data were analyzed with the statistical software Infostat (2018). The species M. fijiensis was identified by PCR with specific primers. In the in vitro growth control assay of black Sigatoka, four strains inhibit growth: DARA33 (Bacillus licheniformis), MTF12 (B. safensis), PMB10 (B. amyloliquefaciens subsp. plantarum) and MAM11 (Leclercia adecarboxylata) and sixteen strains do not inhibit their growth.
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Copyright (c) 2024 Iris Esther Marcano, César Antonio Díaz-Alcántara, Ángel Radhamés Pimentel Pujols, Ángel Felipe Vicioso Alcalá, Pedro Antonio Núñez Ramos
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Revista de Investigación e Innovación Agropecuaria y de Recursos Naturales por Instituto de Investigaciones Agropecuarias y de Recursos Naturales, Facultad de Agronomía, Universidad Mayor de San Andrés se distribuye bajo una Licencia Creative Commons Atribución-NoComercial-CompartirIgual 4.0 Internacional.
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